Next-generation sequencing (NGS) utilizes massively parallel sequencing to generate thousands of megabases of sequence information per day, opening doors to new research studies that were once difficult to accomplish in a practical manner. Powered by semiconductor chips, Ion Torrent™ sequencing technology is simpler, faster, and more cost-effective and scalable than other benchtop next-generation sequencing technologies. Experience the speed, scalability, and simplicity of Ion Torrent™ sequencing.

Ion Proton™ System for Next-Generation Sequencing

测序技术的比较：

https://www.ebi.ac.uk/training/online/course/ebi-next-generation-sequencing-practical-course/what-next-generation-dna-sequencing/improveme

Unlike Illumina and 454, Ion torrent and Ion proton sequencing do not make use of optical signals. Instead, they exploit the fact that addition of a dNTP to a DNA polymer releases an H⁺ ion.

As in other kinds of NGS, the input DNA or RNA is fragmented, this time ~200bp. Adaptors are added and one molecule is placed onto a bead. The molecules are amplified on the bead by emulsion PCR. Each bead is placed into a single well of a slide.

Like 454, the slide is flooded with a single species of dNTP, along with buffers and polymerase, one NTP at a time. The pH is detected is each of the wells, as each H⁺ ion released will decrease the pH. The changes in pH allow us to determine if that base, and how many thereof, was added to the sequence read.

简单的说ion proton 识别碱基是通过ph值，而不是光学信号。

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