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augustus, gene prediction, trainning

时间:2015-11-10 15:50:12      阅读:362      评论:0      收藏:0      [点我收藏+]

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做基因组注释

先用augustus训练,然后再用maker

网址:

http://bioinf.uni-greifswald.de/augustus/

可以在线分析

也可以本地。

在线训练网址:

http://bioinf.uni-greifswald.de/webaugustus/training/create

You have to give a species name(不能有空格!), and a genome file!

关于参考基因组 和cDNA fasta文件的head要求:

  • no whitespaces in the headers
  • no special characters in the headers (e.g. !#@&|;)
  • make the headers as short as possible
  • let headers not start with a number but with a letter
  • let headers contain letters and numbers, only

In the following we give some header examples that will not cause problems:

>entry1
>contig1000
>est20
>scaffold239

 

详细的在线训练指导:

http://bioinf.uni-greifswald.de/webaugustus/trainingtutorial.gsp

如果在线训练基因组大小和cDNA大小均不能超过100M,所以菌类可以用。植物的话还是用本地训练:

/share/bioinfo/zhangxt/software/augustus-2.4/scripts/autoAug.pl --species=Carya --genome=../Carya.fa --cdna=../Carya_400cDNA.fa

如果提示没有加环境变量:

 vi ~/.bash_profile
 export AUGUSTUS_CONFIG_PATH=/share/bioinfo/zhangxt/software/augustus-2.4/config
source ~/.bash_profile
 
 
freemao
FAFU

 

augustus, gene prediction, trainning

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原文地址:http://www.cnblogs.com/freemao/p/4953140.html

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