NgAgo基因编辑技术

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      附录：DNA-guided genome editing using theNatronobacterium gregoryi Argonaute

DNA-guided genome editing using the Natronobacterium gregoryi Argonaute
Feng Gao,Xiao Z Shen,Feng Jiang,Yongqiang Wu & Chunyu Han
AffiliationsContributionsCorresponding author
Nature Biotechnology (2016) doi:10.1038/nbt.3547
Received 03 June 2015 Accepted 21 March 2016 Published online 02 May 2016

     Abstract：The RNA-guided endonuclease Cas9 has made genome editing a widely accessible technique. Similar to Cas9, endonucleases from the Argonaute protein family also use oligonucleotides as guides to degrade invasive genomes. Here we report that the Natronobacterium gregoryi Argonaute (NgAgo) is a DNA-guided endonuclease suitable for genome editing in human cells. NgAgo binds 5′ phosphorylated single-stranded guide DNA (gDNA) of ~24 nucleotides, efficiently creates site-specific DNA double-strand breaks when loaded with the gDNA. The NgAgo–gDNA system does not require a protospacer-adjacent motif (PAM), as does Cas9, and preliminary characterization suggests a low tolerance to guide–target mismatches and high efficiency in editing (G+C)-rich genomic targets.

    Figure 5: NgAgo can be used to edit mammalian genome.                                

        Schematic of the HDR-mediated donor DNA insertion edited by NgAgo-gDNA. The donor is composed of a reporter region and a G418 resistance gene. The latter is directed by a CMV promoter. The reporter is composed of a mRFP gene and an…

参考链接：

[1] 韩春雨：“一鸣惊人”的中国科学家发明世界一流新技术;如何看待颜宁对韩春雨研究成果的评价？

[2] 韩春雨团队在《Nature Biotech》上发表的 NgAgo 基因编辑技术是什么？有何突破？

[3] 中国学者研发出国际一流基因编辑技术; 试读春雨老师新一代基因编辑技术的论文

[4] 水木社区：韩春雨《Nature Biotech》NgAgo 基因编辑技术[知乎] 

[5] http://weibo.com/1656918431/Dwa0H0PFC?type=comment#_rnd1463900877657

[6] http://jinghuasoft.com/smthview.jsp?board=QingJiao&id=348515

[7] http://top10info01.azurewebsites.net/Content.aspx?pid=424620&source=0

NgAgo基因编辑技术

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原文地址：http://www.cnblogs.com/panscience/p/5516927.html